pBINPLUS: An improved plant transformation vector based on pBIN19 |
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| Authors: | Fred A. van Engelen Jos W. Molthoff Anthony J. Conner Jan-Peter Nap Andy Pereira Willem J. Stiekema |
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| Affiliation: | (1) Department of Molecular Biology, Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA Wageningen, The Netherlands;(2) Present address: New Zealand Institute for Crop & Food Research, Private Bag 4704, Christchurch, New Zealand |
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| Abstract: | We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number inE. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes. |
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| Keywords: | transformation vector pBIN19 transgenic plants T-DNA Agrobacterium tumefaciens |
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