Development of Chromosome Segment Substitution Lines Derived from Backcross between Two Sequenced Rice Cultivars, Indica Recipient 93-11 and Japonica Donor Nipponbare |
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Authors: | Wenyin Zhu Jing Lin Dewei Yang Ling Zhao Yadong Zhang Zhen Zhu Tao Chen Cailin Wang |
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Affiliation: | (1) Institute of Food Crops, Jiangsu High Quality Rice Research and Development Center, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China |
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Abstract: | Chromosome segment substitution lines (CSSLs) are powerful tools for detecting and precisely mapping quantitative trait loci (QTLs) and evaluating gene action as a single factor. In this study, 103 CSSLs were produced using two sequenced rice cultivars: 93-11, an elite restorer indica cultivar as recipient, and Nipponbare, a japonica cultivar, as donor. Each CSSL carried a single chromosome substituted segment. The total length of the substituted segments in the CSSLs was 2,590.6 cM, which was 1.7 times of the rice genome. To evaluate the potential application of these CSSLs for QTL detection, phenotypic variations of seed shattering, grain length and grain width in 10 CSSLs were observed. Two QTLs for seed shattering and three for grain length and grain width were identified and mapped on rice chromosomes. The results demonstrate that CSSLs are excellent genetic materials for dissecting complex traits into a set of monogenic loci. These CSSLs are of great potential value for QTL mapping and plant marker-assisted breeding (MAB). |
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Keywords: | Chromosome segment substitution lines Quantitative trait loci Rice SSR marker |
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