Single nucleotide polymorphisms within the promoter region of the rhesus monkey tumor necrosis factor-alpha gene |
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Authors: | Krishna K. Singh Jörg Schmidtke |
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Affiliation: | (1) Institute of Human Genetics, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany |
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Abstract: | The human TNF-alpha gene is characterized by several single nucleotide polymorphisms (SNPs) in its promoter region, in part having been shown to influence TNF-alpha expression and susceptibility to various diseases. The rhesus macaque is widely used as an animal model for a variety of TNF-alpha associated pathological conditions, but little is known about genetic variation within the TNF-alpha promoter region. In order to check for such polymorphisms, primers based on rhesus sequence within 5 UTR were designed and used to amplify a 1 kb product from genomic DNA of 29 animals. Sequencing and cloning revealed a total of 11 polymorphisms leading to five different haplotypes. |
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Keywords: | Tumor necrosis factor-alpha (TNF-alpha) Single nucleotide polymorphisms (SNPs) Animal model Rhesus monkey |
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