凝胶过滤层析参数对家蝇蛋白粗提液分离效果的影响

考察了凝胶过滤介质、层析柱直径、柱床高度和洗脱流速对家蝇Muscadomestica蛋白粗提液分离效果的影响，结果表明：凝胶过滤介质种类、层析柱直径、柱床高度、洗脱流速均能不同程度地影响家蝇蛋白粗提液的分离效果。在实验范围内，选择1.3 cm直径、40 cm柱床高度的Sephadex G-75，以0.4 mL/min的流速洗脱时，对家蝇蛋白粗提液的分离效果比较理想。

Optimization of the gel-filtration chromatography parameters for separating proteins from the crude extracts of housefly

The influences of gel filtration chromatography media, column diameter, column height and flow rate on separation performance of the protein crude extracts of housefly (Musca domestica) were investigated.The results indicated that the all above four factors could affect the separation performance of the crude extracts in gel filtration chromatography by various degrees.In the scope of the present tests, the best separation performance was achieved using Sephadex G-75 (1.3 cm×40 cm) with 0.4 mL/min rate.