A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity |
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Authors: | Yan Fei Yang Wen-Kui Li Xin-Yang Lin Ting-Ting Lun Yan-Ni Lin Feng Lv Shao-Wu Yan Gang-Lin Liu Jun-Qiu Shen Jia-Cong Mu Ying Luo Gui-Min |
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Institution: | Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, People's Republic of China. |
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Abstract: | Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a Schistosoma japonicum GST (SjGST) and a bifunctional 35-mer peptide with SOD and GPX activities. The engineered protein demonstrated SOD, GPX and GST activities simultaneously. This trifunctional enzyme with SOD, GPX and GST activities is expected to be the best ROS scavenger. |
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