Effect of mannitol and glucose-induced osmotic stress on growth,water relations,and solute composition of cell suspension cultures of poplar (Populus deltoides var.Occidentalis) in relation to anthocyanin accumulation |
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Authors: | Ashok Tholakalabavi Janusz J. Zwiazek Trevor A. Thorpe |
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Affiliation: | (1) Department of Forest Science, University of Alberta, T6G 2H1 Edmonton, Alberta, Canada;(2) Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4 Calgary, Alberta, Canada |
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Abstract: | Summary A cell suspension culture of poplar (Populus deltoides (Marsh.) Bartr. var.occidentalis Rydb.), accumulating the anthocyanin pigment, cyanidin 3-glucoside, in the lag phase of culture growth, was subjected to osmotic stress with glucose and mannitol. Osmotic stress treatments resulted in growth suppression and higher anthocyanin accumulation compared with unstressed cells. Both an increase in the proportion of pigmented cells and an increase in the concentration of anthocyanin in the pigmented cells were responsible for high anthocyanin content of cultured cells subjected to osmotic stress. The osmotic stress induced by glucose suppressed growth more than that by mannitol and produced higher anthocyanin levels. Only small amounts of [U-14C]mannitol were taken up and metabolized by the cells. Stressed cells accumulated sugars and free amino acids to a different extent resulting in altered cell sugar-to-amino acid ratios. The accumulation of osmotically active solutes and cell growth suppression may both be responsible for the accumulation of anthocyanin in stressed cells. |
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Keywords: | anthocyanin accumulation free amino acids mannitol metabolism osmotic potential poplar cell suspension soluble sugars water potential |
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