Meiotic chromosome behaviour reflects levels of sequence divergence inSus scrofa domestica satellite DNA |
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| Authors: | M. Jantsch B. Hamilton B. Mayr D. Schweizer |
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| Affiliation: | (1) Department of Cytology and Genetics, Institute of Botany, University of Vienna, A-1030 Vienna, Austria;(2) Department of Animal Breeding and Genetics, University of Veterinary Medicine, A-1030 Vienna, Austria;(3) Present address: Department of Embryology, Carnegie Institution of Washington, 21210 Baltimore, MD, USA |
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| Abstract: | We present a general model for the evolution of chromosome-specific satellite DNA subfamilies.Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac  subgenomes ). We show that the centromeric heterochromatin is characterised by two distinct satellite DNA families designed Mc1 and Ac2. Mc1 is a diverse satellite family of the Mc subgenome of which certain members with a 100 bp repeat unit are found to occur at the pericentromeric regions of each Mc autosome, while others are chromosome-specific, e.g. clone Mc pAv1.5, a higher order repeat variant, which hybridises specifically to chromosome 1. Ac2 is a homogeneous satellite occurring at the subterminal pericentromeric regions of all Ac autosomes. DNA sequence analyses showed that all clones investigated are built up from a 14 bp repeat unit which is highly conserved. In situ hybridisation to meiotic pachytene nuclei revealed a distinct spatial arrangement of the Ac2 centromeric satellite. |
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