Effect of sodium selenite and methyl methanesulfonate or N-hydroxy-2-acetylaminofluorene co-exposure on sister-chromatid exchange production in human whole blood cultures |
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Authors: | James H. Ray Lewis C. Altenburg Maryce M. Jacobs |
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Affiliation: | 1. Medical Genetics Center, The University of Texas Graduate School of Biomedical Sciences, P.O. Box 20334, Astrodome Station, Houston, Texas 77025, USA;2. Department of Biochemistry, The University of Texas System Cancer Center, 6723 Bertner Avenue, Houston, Texas 77030 U.S.A. |
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Abstract: | Sodium selenite (Na2Se03) was tested for its sister-chromatid exchange (SCE)-inducing ability in human whole blood cultures and for the effect of its co-exposure with methyl methanesulfonate (MMS) or N-hydroxy-2-acetyl aminofluorene (N-OH-AAF) on SCE frequency. Long exposure times (77 h and 96 h) to 3.95 × 10-6 M Na2SeO3 resulted in cell death as measured by mitotic indices, but mitotic figures were present after exposure to higher concentrations for a shorter time (19 h). High Na2SeO3 concentrations (7.90 × 10?6 and 1.19 × 10?5 M) resulted in a three-fold increase in the SCE frequency above background level (6–7 SCEs/cell). Exposure of lymphocytes to 1 × 10?4 M MMS for the last 19 h of culture yielded an average SCE frequency of 30.17 ± 0.75 while a similar exposure to 2.7 × 10?5 M N-OH-AAF resulted in 13.61 ± 0.43 SCEs/cell. Simultaneous addition of the high Na2Se03 concentrations and MMS or N-OH-AAF to the cultures resulted in SCE frequencies that were 25–30% and 11–17%, respectively, below the sum of the SCE frequencies produced by the individual compounds. |
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Keywords: | DMBA MMS methyl methanesulfonate N-OH-AAF SCE(s) sisterchromatid exchange(s) |
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