Identification of self-incompatibility-related glycoproteins in styles of apple (Malus x domestica) |
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Authors: | H. Sassa N. Mase H. Hirano H. Ikehashi |
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Affiliation: | (1) Faculty of Horticulture, Chiba University, Matsudo, 271 Chiba, Japan;(2) Department of Molecular Biology, National Institute of Agrobiological Resources, Kannondai, Tsukuba, 305 Ibaraki, Japan |
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Abstract: | In this study, stylar proteins of apple (Malus x domestica) which correlate with known intervarietal incompatibility relationships and have similar characteristics to the S-glycoproteins of Japanese pear (Pyrus serotina) were surveyed by two-dimensional gel electrophoresis (2D-PAGE). Varietal differences were detected in a group of glycoproteins having Mrs and pIs similar to those of the S-glycoproteins of Japanese pear. 2D-PAGE profiles of these glycoproteins were correlated with intervarietal incompatibility relationships. These glycoproteins reacted with antiserum raised against the S4-glycoprotein of Japanese pear, a result suggesting that they may be the products of S-alleles in styles of apple. On the basis of the profiles of the putative S-glycoproteins, S-genotypes were proposed for each of the apple cultivars examined. |
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Keywords: | Self-incompatibility Apple Malus x domestica Two-dimensional gel electrophoresis S-glycoprotein |
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