Electron spectroscopic imaging (ESI) applied to the detection of potential Ca2+-binding sites in plant cells |
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Authors: | Werner Herth Hans-Dieter Reiss |
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Affiliation: | 1. Zellenlehre, Universit?t Heidelberg, Im Neuenheimer Feld 230, D-6900, Heidelberg, Federal Republic of Germany
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Abstract: | The applicability of the electron spectroscopic imaging technique for detection of the intracellular distribution of calcium in plant cells was tested with calyptra cells ofZea mays and with pollen tubes ofLilium longiflorum. After fixation in enhanced Ca2+ levels and embedding in resin, ultrathin sections were analyzed for the elemental distribution. Calcium and phosphorus were enriched in cell wall, plasma membrane, endoplasmic reticulum, mitochondria, and Golgi vesicles, mainly in granular or globular deposits appearing electron dense in transmission electron microscopy. The results demonstrated that the ESI-technique allows exact localization of calcium enrichment relative to specific cell organelles. |
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