Amperometric micro-immunosensor for the detection of tumor biomarker |
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Authors: | Shradha Prabhulkar Subbiah Alwarappan Guodong Liu Chen-Zhong Li |
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Affiliation: | aNanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, 10555 W. Flagler Street, Miami, FL 33174, United States;bDepartment of Chemistry and Molecular Biology, North Dakota State University, 158 Dunbar Hall Fargo, ND 58105-5516, United States |
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Abstract: | In this paper, a highly sensitive, reagentless, electrochemical strategy is reported for the detection of a cancer biomarker-Vascular Endothelial Growth Factor (VEGF). Disc shaped carbon fiber microelectrodes were used as the immunosensor platform. Ferrocene monocarboxylic acid labeled anti-VEGF was covalently immobilized on the microelectrode surface using a Jeffamine cross-linker. The formation of immunocomplexes leads to a decrease in the electrochemical signal of ferrocene monocarboxylic acid owing to increased spatial blocking of microelectrode surface. These signal changes enable quantitative detection of VEGF in solution. Voltammetric measurements were conducted to evaluate the interfacial immunoreactions and to quantitatively detect VEGF biomarker. The proposed immunosensing strategy allows a rapid and sensitive means of VEGF analysis with a limit of detection of about 38 pg/mL. This opens up the possibility of employing these electrodes for various single cell analysis and clinical applications. Further, experimental conditions such as concentration of the immobilized antibodies and incubation period were optimized. Following this, the stability and specificity of the immunosensors were also evaluated. |
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Keywords: | Electrochemical biosensors Cancer Microelectrodes Immunosensor Biomarkers Vascular Endothelial Growth Factor |
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