Molecular cloning and characterization of the trpC gene from Penicillium chrysogenum |
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Authors: | Flora Sá nchez, Angeles Touriñ o, Susana Traseira, Agustí n Pé rez-Aranda, Ví ctor Rubio Miguel A. Peñ alva |
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Affiliation: | (1) Departamento de Genética Molecular Antibióticos S.A., Bravo Murillo 38, E-28015 Madrid, Spain |
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Abstract: | Summary We cloned the Penicillium chrysogenum trpC gene from a genomic library by complementation of an Escherichia coli trpC mutant lacking phosphoribosylanthranilate isomerase activity. The gene ecodes a 2.7 kb poly(A)+ RNA. We localized the gene by sequence analysis in a 2.9 kb DNA insert found in the smallest plasmid selected from the library. Sequence data strongly suggest that the organization of the gene is similar to that described in other Ascomycetes. We found that a DNA fragment which codes only for the carboxy-terminal protion of the polypeptide is sufficient for complementation of the E. coli trpC9830 mutation. |
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Keywords: | Genomic library Complementation trpC gene mRNA Penicillium chrysogenum |
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