| 半夏凝集素的分离纯化和在植物中的分布 |
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| 引用本文： | 王克夷,郭锰.半夏凝集素的分离纯化和在植物中的分布[J].中国生物化学与分子生物学报,1993,9(5):544-548. |
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| 作者姓名： | 王克夷 郭锰 |
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| 作者单位： | 中国科学院上海生物化学研究所，中国科学院上海生物化学研究所 上海 200031，上海 200031 |
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| 摘 要： | 从半夏(Pinellia ternata)块根中,经95％硫酸铵沉淀和固定化猪甲状腺球蛋白柱亲和层析分离得到一种凝集素,称为半夏凝集素(PTL)。凝胶过滤、SDS-PAGE和免疫双扩散鉴定PTL是均一的样品,分子量约44kD,由四个约12kD的亚基组成。PTL主要存在于半夏的块茎中,茎中也有少量存在。
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| 关 键 词： | 半夏凝集素 甘露聚糖结合活性 分离纯化 |
| 收稿时间： | 1993-10-20 |
Purification of Lectin from Pinellia Ternata |
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| Wang,Ke-yi Guo,Meng.Purification of Lectin from Pinellia Ternata[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(5):544-548. |
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| Authors: | Wang Ke-yi Guo Meng |
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| Institution: | (Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031 |
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| Abstract: | Pinellia ternata lectin (PTL) has been purified from root tuber juice of Pinellia ternata by precipitation with 95% saturation of ammonium sulfate and affinity chromatography on immobilized porcine thyroglobulin. Purified PTL was identified to be homogeneous by gel filtration, SDS-PAGE and double immunodiffusion. Mr of PTL was about 44kD and it was consisted of four subunits. Majority of PTL occured in root tuber, but there was still small amount in stalk. |
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| Keywords: | Pinellia ternata lectin (PTL) Mannan-binding activity Affinity chromatography |
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