In VitroAssays for Activities of Retroviral Integrase

Integration of retroviral DNA, an essential step during the retroviral life cycle, is mediated by the viral protein integrase. Simplein vitroassays for measuring integrase activities are described, including catalysis (3′-end processing, 3′-end joining, disintegration), juxtaposition of viral DNA ends, DNA binding, and target site selection. The described assays will be useful in elucidating the molecular mechanism of retroviral integration and screening for integrase inhibitors as potential anti-retroviral drugs.