Effect of chloral hydrate on in vivo KCl-induced striatal dopamine release in the rat |
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Authors: | Han-Tong Chen Sathasiva B Kandasamy |
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Institution: | (1) Department of Radiation, Pathophysiology, and Toxicology, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, 20889-5603 Bethesda, Maryland |
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Abstract: | The release of striatal dopamine (DA) and its metabolites in response to locally-induced K+ depolarization was investigated in vivo in chloral hydrate-anesthetized and freely moving rats. KCl at concentrations of
30, 50, and 100 mM induced significant dose-dependent increases in extracellular DA overflow in both chloral hydrate-anesthetized
and freely moving rats (P<0.05). Extracellular levels of dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and
5-hydroxyindoleacetic acid (5-HIAA) were decreased. The DA overflow in response to 30 mM KCl stimulation in anesthetized rats
was significantly greater than that in freely moving rats (P<0.05). In addition, chloral hydrate anesthesia resulted in a
significant decrease in extracellular levels of DOPAC and significant increases in extracellular levels of HVA and 5-HIAA
in comparison with freely moving rats (P<0.05). Furthermore, the basal level of extracellular HVA in chloral hydrateanesthetized
rats was significantly higher than that in freely moving rats. These results suggest that chloral hydrate anesthesia could
have significant effects on the pharmacological response of the striatal dopaminergic neurons. |
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Keywords: | Microdialysis anesthesia dopamine chloral hydrate striatum |
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