Programmed cell death induced by (β-d-galactosyl)3 Yariv reagent in Nicotiana tabacum BY-2 suspension-cultured cells |
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| Authors: | Inê s Chaves,Ana Paula Regalado ,Ming Chen,Câ ndido Pinto Ricardo, Allan M. Showalter |
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| Affiliation: | Instituto de Tecnologia Química e Biológica, Plant Biochemistry Laboratory, Apartado 127, 2780-901 Oeiras, Portugal;Ohio University, Department of Environmental and Plant Biology, Molecular and Cellular Biology Program, Athens, OH 45701-2979 USA;Instituto Superior de Agronomia, 1349-017 Lisboa Codex, Portugal |
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| Abstract: | Programmed cell death (PCD) is involved in plant development and pathogen defence and can be triggered in vitro by several biotic and abiotic stimuli. In this report ( β - d -galactosyl)3 Yariv reagent, a chemical that specifically binds to arabinogalactan-proteins (AGPs), completely inhibited cell growth and induced PCD in tobacco BY-2 suspension cultured cells. Analysis of DNA from these cells, by agarose gel electrophoresis, revealed a DNA ladder consisting of multimers of 140–170 bp, similar to apoptotic animal DNA internucleosomal fragmentation. Complementary morphological studies revealed additional PCD characteristics in the Yariv-treated BY-2 cells, including cell shrinkage and cytoplasmic condensation. These studies demonstrate the usefulness of BY-2 cells as a model plant PCD system and confirm a link between AGPs and PCD. |
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