Comparison of the homologous SfeI and LlaBI restriction-modification systems |
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Authors: | Okhapkina S S Netesova N A Golikova L N Seregina E V Sosnovtsev S V Abdurashitov M A Degtiarev S Kh |
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Affiliation: | Institute of Molecular Biology, Vektor State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk Region, 633159 Russia. sso@lae.ma.nsc.ru |
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Abstract: | A fragment containing the SfeI restriction-modification system (RMS) operon was cloned from a Streptococcus faecalis SE72 plasmid. Nucleotide sequence analysis revealed its high (99.2%) homology to the operon for Lactococcus lactis subsp. cremoris W56 LlaBI RMS recognizing the same site, 5'-CTRYAG-3'. A substantial difference was that SfeI RMS operon had an additional 198-bp fragment and a larger gene for the putative control protein. No homology was observed between operon-flanking sequences of the two closely related species, suggesting horizontal transfer of the operon. |
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