13C NMR analysis of methionine sulfoxide in protein. |
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| Authors: | J S Cohen J Yariv A J Kalb L Jacobson Y Shechter |
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| Affiliation: | Department of Biophysics, Isotope Research and Organic Chemistry, The Weizmann Institute of Science, Rehovot Israel |
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| Abstract: | The 13C epsilon NMR signal of methionine sulfoxide is 22.6 ppm downfield from that of methionine. This affords a method by which the extent of methionine oxidation can be determined in intact protein. We demonstrate the utility of this approach with beta-galactosidase enriched with 13C in its methionine methyls. |
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