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Role of metallothionein isoforms in bone formation processes in rat marrow mesenchymal stem cells in culture |
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| Authors: | Yoshiko Dohi Hideki Shimaoka Masako Ikeuchi Hajime Ohgushi Kunio Yonemasu Takeshi Minami |
| Affiliation: | (1) Department of Public Health, Nara Medical University, 840 Shijo-cho, 634-8521 Kashihara City, Nara, Japan;(2) Department of Oral and Maxillofacial Surgery, Nara Medical University, 840 Shijo-cho, 634-8521 Kashihara City, Nara, Japan;(3) Department of Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Amagasaki Site 3-11-46 Nakouji, 661-0974 Amagasaki City, Hyogo, Japan;(4) Present address: Department of Life Sciences, School of Science and Technology, Kinki University, 577-8502 Higashi-osaka, Japan |
| Abstract: | Temporal changes in mRNAs for metallothionein (MT) isoforms in subcultures of rat marrow mesenchymal stem cells (MSCs) after treatment with dexamethasone were investigated. Both MT-1 and MT-2 mRNA expression in the cultured MSCs with dexamethasone showed maximum levels at d 1, whereas ALP and osteocalcin mRNAs peaked at d 12. MT-3 mRNA was not detected in the cultured MSCs at any time. The expression level of MT-2 mRNA at d 1 was 9.4-fold higher than that of MT-1 mRNA. Finally, osteoblast differentiation and mineralization of MSCs at d 14 was inhibited by the addition of a common antisense oligonucleotide for both MT-1 and MT-2 in the culture medium during the first 4 d. The results suggest that the large amounts of MT-2 are produced in the early stage of subculture of MSCs, and this might regulate their differentiation. |
| Keywords: | Marrow mesenchymal stem cells metallothionein isoforms osteoblast differentiation dexamethasone alkaline phosphatase |
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