KINETICS OF NEUTROPHILIC GRANULOCYTES IN THE BLOOD OF RATS |
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Authors: | D Gerecke B Schultze W Maurer |
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Institution: | Institut für Medizinische Strahlenkunde, Universität Würzburg, D 87 Würzburg, Germany |
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Abstract: | The kinetics of neutrophilic granulocytes in the blood of rats were investigated using in vivo 3H-TdR labelling and autoradiography. The radioactive precursor was administered by single injection, repeated injections at 5 hr intervals and continuous infusion. The appearance of labelled granulocytes in the blood and in the sputum was recorded up to 120 hr after tracer application. In contrast to results after a single injection of 3H-TdR, complete labelling of the blood granulocyte pool was achieved when the DNA precursor was given by continuous infusion or four repeated injections at 5 hr intervals. In the latter experiments, an exponential replacement of unlabelled blood granulocytes by labelled granulocytes could be demonstrated, the mean intravascular half-life being 5·7 hr. This figure is in good agreement with values obtained by isotopic techniques in other mammalian species. |
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