Comparison of different cationic probes for electron microscopic visualization of bacterial polyanionic capsular material |
| |
Authors: | Dr Mario Jacques Bernadette Foiry |
| |
Institution: | (1) Department of Biochemistry, Microbiology and Nutrition, University of New England, 2351 Armidale, Australia;(2) Present address: School of Business & Technology, Macarthur Institute of Higher Education, 2360 Campbelltown, Australia |
| |
Abstract: | We have isolated from the ovine rumen eight bacterial strains belonging to the speciesButyrivibrio fibrisolvens. DNA hybridization studies showed that the eight strains could be divided into four homology groups, of which none was closely related to the type strain ATCC 19171. Measurement of cross-hybridization between selected pairs of bacterial strains showed that DNA types which produced low, but significant, cross-hybridization on dot-blots were able to form heteroduplexes with between 8.4% and 32.9% of the efficiency of homoduplex formation. Thermal denaturation of the same heteroduplexes resulted in Tm values 6.4–7.5°C lower than those of the homologous duplexes formed under the same conditions. In some cases, hybridization between strains was below the level of reliable measurement. Similar experiments with ten recently isolated strains ofBacteroides ruminicola sub-sp.brevis revealed a similar degree of genetic divergence between isolates. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|