| GFP基因转化香樟胚性愈伤组织的研究 |
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| 引用本文： | 杜丽,曾晓慧,周索,包满珠. GFP基因转化香樟胚性愈伤组织的研究[J]. 西北植物学报, 2008, 28(3): 465-469 |
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| 作者姓名： | 杜丽 曾晓慧 周索 包满珠 |
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| 作者单位： | 1. 南阳师范学院,生命科学与技术学院,河南南阳,473000
2. 华中农业大学,园艺林学学院,教育部园艺植物生物学重点实验室,武汉,430070 |
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| 基金项目： | 河南省自然科学基金资助(0611033300);南阳师范学院高层次人才科研启动费资助 |
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| 摘 要： | 以香樟胚性愈伤组织作为受体,利用根癌农杆菌介导法进行了绿色荧光蛋白基因(GFP)的遗传转化研究。经农杆菌侵染后的胚性愈伤组织通过共培养、选择培养后获得抗性愈伤组织和体胚,对抗性愈伤组织及体胚的诱导过程进行了GFP荧光检测。结果表明,GFP基因能在抗性愈伤组织和体胚中强烈表达,证明GFP基因能够在香樟遗传转化中得到应用。对抗性愈伤组织的PCR检测初步证实外源GFP基因已整合到香樟胚性愈伤组织的基因组中。
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| 关 键 词： | GFP基因 香樟 根癌农杆菌 PCR |
| 文章编号： | 1000-4025(2008)03-0465-05 |
| 修稿时间： | 2007-10-16 |
Transfer of Green Fluorescent Protein Gene into Embryogenic Calluses of Cinnamomum camphora |
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| DU Li,ZENG Xiao-hui,ZHOU Suo,BAO Man-zhu. Transfer of Green Fluorescent Protein Gene into Embryogenic Calluses of Cinnamomum camphora[J]. Acta Botanica Boreali-Occidentalia Sinica, 2008, 28(3): 465-469 |
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| Authors: | DU Li ZENG Xiao-hui ZHOU Suo BAO Man-zhu |
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| Abstract: | In order to confirm its practical value of the green fluorescent protein(GFP),a visual marker,in camphor tree(Cinnamomum camphora) transformation,Agrobacterium tumefaciens-mediated transformation by using embryogenic calluses as explants was investigated.GFP detection was conducted during the transformation and strong GFP expression was observed in putative transformants which indicated that GFP could be used in camphor tree transformation.Results of PCR proved preliminarily that the GFP gene had integrated into C.camphora embryogenic calluses genome. |
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| Keywords: | green fluorescent protein gene Cinnamomum camphora Agrobacterium tumefaciens PCR |
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