Optimization of Conditions for Storage and Cultivation of the Fungus Claviceps sp., a Producer of the Ergot Alkaloid Agroclavine |
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Authors: | Boichenko L V Zelenkova N F Arinbasarov M U Reshetilova T A |
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Institution: | (1) Russian Academy of Sciences, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino, Moscow oblast, 142290, Russia |
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Abstract: | Conditions of agroclavine biosynthesis by the mutant Claviceps sp. strain c106 were studied. The content of agroclavine was maximum (1.5–2 g/l) on days 15–16 of cultivation in the complex medium T25, containing sucrose, citric acid, and yeast extract. Agroclavine was the major component of the alkaloid fraction (90–95%). Storage of the culture at –70°C in T25 supplemented by 7% glycerol provided a stable level of alkaloid formation. |
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