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Cotransduction of his and trp loci by phage SV1 in Streptomyces venezuelae
Authors:C Stuttard
Institution:Microbiology Department, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7
Abstract:Abstract A new tryptophan auxotroph of Streptomyces venezuelae was isolated by transduction of a histidine mutant to His+ after hydroxylamine mutagenesis of the transducing lysate. Cotransduction of at least two his genes and two trp genes was then demonstrated. One trp and two his markers were not cotransducible with this gene cluster, but the separate trp marker, trp -4, was cotransducible with a nic marker. These results are compatible with the distribution of similar markers on the conjugational linkage map of S. coelicolor A3(2), and so support the hypothesis of conservation of linkage relationships among putative homologous loci within the genus Streptomyces .
Keywords:Localized mutagenesis              S  coelicolor            linkage map
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