| 一株微生物菌株催化水解拆分外消旋6-羟基-8-氯辛酸乙酯 |
| |
| 引用本文： | YANG Qian,倪晔,ZHENG Pu,孙志浩.一株微生物菌株催化水解拆分外消旋6-羟基-8-氯辛酸乙酯[J].生物加工过程,2008,6(4):13-18. |
| |
| 作者姓名： | YANG Qian 倪晔 ZHENG Pu 孙志浩 |
| |
| 作者单位： | 江南大学,工业生物技术教育部重点实验室,生物催化研究室,无锡,214122 |
| |
| 基金项目： | 国家自然科学基金资助项目
,
国家"973"资助项目
,
长江学者和创新团队发展计划资助项目 |
| |
| 摘 要： | 从土壤中筛选到一株能够拆分外消旋的硫辛酸中间体6-羟基-8-氯辛酸乙酯的微生物菌株T4，并对其拆分反应条件进行了优化，确定了最适反应温度为30℃，最适pH为7．0，最适摇床转速为170r／min，确定了有效表面活性剂为CTAB，在此优化条件下反应8h，得到（R）-6-羟基-8-氯辛酸乙酯的对映体过量值（e．e．）为92．8％，产率为26．3％。该研究为（R）-硫辛酸的制备提供了一条可行的途径。
|
| 关 键 词： | 短杆菌 硫辛酸 (R)-6-羟基-8-氯辛酸乙酯 拆分 |
Resolution of racemic ethyl 6-hydroxy-8-chlorocaprylate by hydrolysis with whole cells of bacteria from soil |
| |
| YANG Qian,NI Ye,ZHENG Pu,SUN Zhi-hao.Resolution of racemic ethyl 6-hydroxy-8-chlorocaprylate by hydrolysis with whole cells of bacteria from soil[J].Chinese Journal of Bioprocess Engineering,2008,6(4):13-18. |
| |
| Authors: | YANG Qian NI Ye ZHENG Pu SUN Zhi-hao |
| |
| Institution: | ( Key Laboratory of Industrial Biotechnology of the Ministry of Education, Laboratory of Biocatalysis, Jiangnan University, Wuxi 214122, China) |
| |
| Abstract: | A strain T4 was screened from soil to catalyze the resolution of racemic ethyl 6-hydroxy-8-chlorocaprylate and the condition of the whole-cell-catalyzed resolution was investigated. The result showed that the optimum reaction conditions were determined as the temperature of 30 ℃, buffer pH 7.0, shaking rate of 170 r/min with CTAB as surfactant. Under the optimal condition, product enantiomeric excess of 92. 8% was achieved with a yield of 26.3% after 8 h reaction. |
| |
| Keywords: | rod bacteria lipoic acid ethyl 6-hydroxy-8-chlorocaprylate resolution |
| 本文献已被 维普 万方数据 等数据库收录！ |
