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Electrophoretic karyotypes of some related Mucor species
Authors:Ágnes Nagy  Zsuzsanna Palágyi  Mária Vastag  Lajos Ferenczy  Csaba Vágvölgyi
Institution:(1) Department of Microbiology, Faculty of Sciences, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary;(2) HAS-JATE Microbiological Research Group, Department of Microbiology, Faculty of Sciences, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary;(3) Department of Microbiology, Faculty of Sciences, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary; Author for correspondence)
Abstract:Contour clamped homogeneous electric field (CHEF) gel electrophoresis was used to obtain electrophoretic karyotypes from nine Mucorstrains representing five different species (M. bainieri, M. circinelloides, M. mucedo, M. plumbeus and M. racemosus). The chromosomal banding patterns revealed high variability among the isolates. The sizes of the DNA in the Mucor chromosomes were estimated to be between 2.5 and 8.7 Mb. The total genome sizes were calculated to be between 30.0 and 44.7 Mb. The applicability of these electrophoretic karyotypes for the investigation of genome structure, for strain identification and for species delimitation is considered.
Keywords:CHEF  chromosome-length polymorphism  electrophoretic karyotyping  Mucor
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