Exposure of human leukemic cells to direct electric current |
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Authors: | Venicio F Veiga Leonardo Nimrichter Cesar A Teixeira Marcelo M Morales Celuta S Alviano Marcio L Rodrigues Carla Holandino |
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Institution: | 1. Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, CCS. Bloco K. Segundo Andar, Sala 50, 21941-590, Ilha do Fund?o, Rio de Janeiro, Brazil 2. Departamento de Medicamentos-Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, CCS. Bloco K. Segundo Andar, Sala 50, 21941-590, Ilha do Fund?o, Rio de Janeiro, Brazil 3. Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS. Bloco K. Segundo Andar, Sala 50, 21941-590, Ilha do Fund?o, Rio de Janeiro, Brazil
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Abstract: | Treatment with direct electric current (DC) influences the growth of several cancer cells. In this work, we evaluated the
effects of DC treatment on the human leukemic cell line HL60. Human cells were separately treated in the presence of the cathode
or the anode or without contact with the electrodes. In all systems, DC-treated cells presented an impaired ability to proliferate.
Growth inhibition was dependent on the generation of soluble products of electrolysis. Cathodic treatment of HL60 cells predominantly
induced lysis, whereas treatment without contact with electrodes did not induce alterations in cell viability. In contrast,
cell stimulation by the anode resulted in irreversible membrane damage, as demonstrated by trypan blue and 7-aminoactinomycin
staining. Analysis of these cells by transmission electron microscopy indicated that necrosis is a major mechanism inducing
cell death. In addition, apoptotic-like cells were observed under light microscopy after anodic treatment. Accordingly, DNA
from anodic-treated cells presented a typical pattern of apoptosis. Apoptotic cell death was only generated after the treatment
of HL60 cells in conditions in which the generation of chloride-derived compounds was favored. These results indicate that
the nature of the products from cathodic or anodic reactions differently influences the mechanisms of cell death induced by
DC-derived toxic compounds. |
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Keywords: | Direct electric current human leukemic cells tumor growth apoptosis necrosis |
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