Genetic transformation of selected mature cork oak (Quercus suber L.) trees |
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| Authors: | R. Álvarez P. Alonso M. Cortizo C. Celestino I. Hernández M. Toribio R. J. Ordás |
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| Affiliation: | (1) Department of Biología de Organismos y Sistemas, University of Oviedo, C/. Catedrático Rodrigo Uría s/n, 33011 Oviedo, Spain;(2) Instituto Madrileño de Investigación Agraria y Alimentaria (IMIA), Finca !["ldquo"]("/content/d37x80qeuf6wnlgh/xlarge8220.gif") El Encín!["rdquo"]("/content/d37x80qeuf6wnlgh/xlarge8221.gif") , Aptdo. 127, Alcalá de Henares, 28080 Madrid, Spain |
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| Abstract: | A transformation system for selected mature cork oak (Quercus suber L.) trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses were inoculated with A. tumefaciens strains EHA105, LBA4404 or AGL1 harbouring the plasmid pBINUbiGUSint [carrying the neomycin phosphotransferase II (nptII) and  -glucuronidase (uidA) genes]. The highest transformation efficiency (4%) was obtained when freshly isolated explants were inoculated with A. tumefaciens strain AGL1. Evidence of stable transgene integration was obtained by PCR for the nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos were germinated and successfully transferred to soil.Abbreviations BA N6-Benzyladenine - GUS -Glucuronidase - MSSH Expression-proliferation medium - NAA  -Naphthaleneacetic acid - nptII Neomycin phosphotransferase gene - uidA -Glucuronidase gene |
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| Keywords: | Agrobacterium Cork oak Fagaceae Somatic embryogenesis Tree genetic transformation |
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