Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803 |
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Authors: | Ajlani Ghada Vernotte Claudie |
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Affiliation: | (1) Centre de Génétique Moléculaire du Centre National de la Recherche Scientifique, associé à l', Université Pierre et Marie Curie, Photosynthèse Bactérienne, Bât. 24, 91198 Gif-sur-Yvette, Cedex, France;(2) Present address: C2, 17 rue des Martyrs, 38054 Grenoble Cedex 09, France |
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Abstract: | A mutant strain of the cyanobacterium Synechocystis PCC 6803, called PAL, (PC-, apcAB, apcE), lacking phycocyanin, allophycocyanin and the core-membrane linker (Lcm), was constructed. The strain was characterized by absorption and fluorescence spectroscopy. The mutant compensates for the absence of the major PS II antenna by increasing its PS II / PS I ratio. It is stable and grows well albeit more slowly than wild type. |
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Keywords: | apc genes fluorescence gene inactivation photosystems phycobiliproteins Synechocystis |
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