Cardiac mitochondrial ATP-sensitive potassium channel is activated by nitric oxide in vitro |
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| Authors: | Ljubkovic Marko Shi Yang Cheng Qunli Bosnjak Zeljko Jiang Ming Tao |
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| Affiliation: | Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. |
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| Abstract: | Previous observations on the activation of the mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) by nitric oxide (NO) in myocardial preconditioning were based on indirect evidence. In this study, we have investigated the direct effect of NO on the rat cardiac mitoK(ATP) after reconstitution of the inner mitochondrial membranes into lipid bilayers. We found that the mitoK(ATP) was activated by exogenous NO donor S-nitroso-N-acetyl penicillamine or PAPA NONOate. This activation was inhibited by mitoK(ATP) blockers 5-hydroxydecanoate or glibenclamide. Our observations confirm that NO can directly activate the cardiac mitoK(ATP), which may underlie its contribution to myocardial preconditioning. |
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| Keywords: | mitoKATP, mitochondrial ATP-sensitive potassium channel NO, nitric oxide IMM, inner mitochondrial membrane OMM, outer mitochondrial membrane SNAP, S-nitroso-N-acetyl penicillamine PPN, PAPA NONOate GLIB, glibenclamide 5-HD, 5-hydroxydecanoate SMP, submitochondrial particles |
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