The occurrence of aerolysin-positive Aeromonas spp. and their cytotoxicity in Norwegian water sources |
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Authors: | Ørmen Ø Østensvik Ø |
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Affiliation: | The Norwegian School of Veterinary Science, Department of Pharmacology, Microbiology and Food Hygiene, Oslo, Norway |
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Abstract: | Aims: The aim of the study was to investigate the occurrence of Aeromonas spp. and their aerolysin status in Norwegian natural water sources. Methods and Results: Seventy-one samples from 33 Norwegian water sources were examined for the presence of Aeromonas spp. From most of the sample sites, the strains were isolated on blood-ampicillin-agar and Difco Aeromonas agar simultaneously. The majority of the samples (73/77) contained Aeromonas spp., with an average of 35–100 cfu 100 ml–1. The highest counts were found in faecally-contaminated water. Using PCR, 445 isolates were screened for the presence of aerolysin, and 79% of them were found to be carriers of the aerolysin gene. A selection of the isolated strains was tested on Vero cell cultures and 83% of them showed cytotoxicity. Conclusions: There is widespread occurrence of aerolysin-positive cytotoxic Aeromonas spp. in many different Norwegian natural waters, including drinking water sources. Significance and Impact of the Study: The widespread occurrence of potentially-pathogenic Aeromonas spp. in the environment demands that these bacteria should not be ignored in drinking water supplies and in the food industry. |
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