Fast separation of 16 seizure drug substances using non-aqueous capillary electrophoresis |
| |
Authors: | Bjørnsdottir I Hansen S H |
| |
Institution: | Department of Analytical and Pharmaceutical Chemistry, The Royal Danish School of Pharmacy, Copenhagen. ib@mail.dfh.dk |
| |
Abstract: | A fast and simple method for separation of 16 seizure drug substances using capillary electrophoresis in a non-aqueous separation medium is described. The separation medium consists of a mixture of acetonitrile, methanol and glycerol with ammonium acetate/acetic acid as the electrolyte. The analytes are detected by UV detection at 214 nm. Injection from the detection end (8.5 cm to detector) combined with the usage of a short capillary (32.5 cm total length) makes it possible to separate all 16 amines within 2 min. The choice of solvents, electrolytes and viscosity increasing additives are discussed with special emphasis to their influence on the separation selectivity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|