Identification and characterization of flavonoids in the root exudate of Robinia pseudoacacia |
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Authors: | Petra Scheidemann A Wetzel |
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Institution: | Philipps-Universit?t Marburg, FB Biologie, Karl-von-Frisch-Strasse, D-35032 Marburg, Germany, DE
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Abstract: | Eight compounds exuded from young roots of black locust (Robinia pseudoacacia) were separated by two-dimensional HPTLC, by HPLC and GC, and were identified by spectroscopic methods (ultraviolet/visible
spectroscopy and mass spectrometry) as 4′,7-dihydroxyflavone, apigenin, naringenin, chrysoeriol and isoliquiritigenin. Structural
assignments were confirmed by comparison with authentic standards. The capacity to induce β-galactosidase activity in Rhizobium sp. NGR234 containing a nod box::lacZ fusion on plasmid pA27 identified these flavonoids and the chalcone as nod gene inducers. This indicates the important role of these compounds in nodulation of this legume tree.
Received: 26 July 1996 / Accepted: 9 September 1996 |
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Keywords: | Robinia pseudoacacia Rhizobium sp NGR234 Root exudate Flavonoids nod Gene induction |
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