Identification of somatogenic binding sites in liver microsomes from normal mice and transgenic mice expressing human growth hormone gene. |
| |
| Authors: | R C Aguilar H N Fernández J M Dellacha R S Calandra A Bartke D Turyn |
| |
| Affiliation: | Instituto de Química y Fisicoquimica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina. |
| |
| Abstract: | Somatogenic binding sites were detected and characterized in microsomal preparations from livers of normal mice and mice expressing metallothionein-I/hGH (mMT/hGH) hybrid gene, using 125I-labelled bovine or human GH, or a photoreactive derivative of hGH (125I-AP-hGH1). Specific binding of 125I-bGH was detected in liver microsomes from both normal and transgenic mice with an apparent Kd of 2 nM. 125I-hGH was partially displaced by bGH. 125I-AP-hGH1 was covalently bound to the microsomal preparations, and bGH prevented the formation of the 130 kDa species with no appreciable effect on 63 kDa and 70 kDa lactogenic complexes. |
| |
| Keywords: | |
|
|
