A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers |
| |
Authors: | Qing-Song Shao Qiao-Sheng Guo Yan-Ming Deng Hai-Peng Guo |
| |
Institution: | a Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, PR China;b Zhejiang A & F University, Hangzhou 311300, PR China;c Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China |
| |
Abstract: | The diversity and genetic relationship among 29 populations of Chrysanthemum morifolium, one of Chrysanthemum indicum and one of Chrysanthemum nankingense from China were analyzed using morphological traits and molecular markers. Twenty morphological traits were scored as well as 182 ISSR marker-fragments, as amplified by 22 primers the percentage of polymorphic bands (PPB): 81.87%], and 243 SRAP marker-fragments as generated by 26 primer pairs (PPB: 75.72%). Mantel’s test indicated significant correlation (r = 0.624) of morphological trait and SRAP. By contrast, the morphological trait showed low correlation with ISSR (r = 0.246). Cluster analysis showed groupings of the accessions according to all four methods correlated well with their geographic region of origin, and most populations from the south of China were classified into one cluster and most populations from the north of China were classified into another cluster. Finally, an appropriate strategy for conserving the C. morifolium germplasm was proposed. |
| |
Keywords: | Chrysanthemum morifolium Genetic diversity Morphological traits ISSR SRAP |
本文献已被 ScienceDirect 等数据库收录! |
|