Quantification of carbonic anhydrase III and myoglobin in different fiber types of human psoas muscle |
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Authors: | A Zheng P Rahkila J Vuori S Rasi T Takala H K Väänänen |
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Institution: | (1) Department of Anatomy, University of Oulu, Kajaanintie 52 A, SF-90220 Oulu, Finland;(2) Rehabilitation and Physical Exercise Center, SF-41350 Laukaa as, Finland;(3) Deaconess Institute of Oulu, SF-90100 Oulu, Finland |
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Abstract: | Summary Carbonic anhydrase (CA III) and myoglobin contents from isolated human muscle fibers were quantified using a sensitive time-resolved fluoroimmunoassay. Human psoas muscle specimens were freeze-dried, and single fibers were dissected out and classified into type I, IIA and IIB by myosin ATPase staining. Fiber typing was further confirmed by SDS-PAGE. CA III and myoglobin were found in all fiber types. Type I fibers contained higher concentrations of CA III and myoglobin than type IIA and IIB fibers. The relative concentrations of CA III in type IIA and IIB fibers were respectively 24% and 10% of that in type I fibers. The relative concentrations of myoglobin in type IIA and IIB fibers were 60% and 28% of that in type I fibers. Anti-CA III immunoblotting results from fiber-specific pooled samples agreed well with quantitative measurements. The results indicate that CA III is a more specific marker than myoglobin for type I fibers. |
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