Decrease of dehydrogenase activity of cerebral glyceraldehyde-3-phosphate dehydrogenase in different animal models of Alzheimer's disease |
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| Authors: | Irina N. Shalova Katerina Cechalova Zuzana Rehakova Petya Dimitrova Elisa Ognibene Antonio Caprioli Elena V. Schmalhausen Vladimir I. Muronetz Luciano Saso |
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| Affiliation: | 1. School of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Leninskie Gory, Moscow, 119992, Russia;2. Department of Human Physiology and Pharmacology “Vittorio Erspamer”, University of Rome “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy;3. Behavioural Pharmacology Lab, Central and Peripheral nervous system, Sigma-Tau SpA, Via Pontina Km 30.4, Pomezia, Italy;4. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory, Moscow, 119992, Russia |
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| Abstract: | Recently, a relationship between glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the β-amyloid precursor protein (βAPP) in relationship with the pathogenesis of Alzheimer's disease (AD) has been suggested. Therefore, we studied the specific activity of GAPDH in the different animal models of AD: transgenic mice (Tg2576) and rats treated with β-amyloid, or thiorphan, or lipopolysaccharides (LPS) and interferon γ (INFγ). We observed that GAPDH activity was significantly decreased in the brain samples from TG mice. The injection of β-amyloid, or thiorphan, an inhibitor of neprilysin involved in β-amyloid catabolism, in rat brains resulted in a pronounced reduction of the enzyme activity. The infusion of LPS and IFNγ, which can influence the progression of the AD, significantly reduced the enzyme activity. |
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| Keywords: | Glyceraldehyde-3-phosphate dehydrogenase Catalytic activity Alzheimer disease Animal models |
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