PRODUCTION OF BIOLOGICALLY ACTIVE HUMAN RELA (p65) IN BACULOVIRUS-INFECTED INSECT CELLS |
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| Authors: | MARIE CHABOT-FLETCHER MAUREEN L. HO JOHN J. BRETON CHARLES R. HANNING BERNARD Y. AMEGADZIE |
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| Affiliation: | 1. Department of Immunopharmacology and, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939, U.S.A.;2. Department of Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939, U.S.A.;1. Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University, Cheongju 28644, Republic of Korea;2. KBNP Technology Institute, KBNP Inc., Anyang 14059, Republic of Korea;1. Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) University of Applied Sciences, Giessen, Germany;2. Justus-Liebig-University Giessen, Faculty of Biology and Chemistry, Giessen, Germany |
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| Abstract: | A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF-κB/Rel family of proteins. Infection of Spodoptera frugiderda insect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting using RelA-specific antisera and by electrophoretic mobility shift assays. The recombinant protein bound specifically to an oligonucleotide containing the NF-κB consensus motif but not to that containing the unrelated Oct-1 consensus motif. Thus insect cell-derived RelA possess properties similar to the native protein and may be used in physical, biochemical, and pharmacological studies. |
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