INDEPENDENT HIGH-FREQUENCY OSCILLATIONS IN THE AMOUNTS OF INDIVIDUAL ISOZYMES OF LACTATE DEHYDROGENASE IN HL60 CELLS |
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| Authors: | G. M. N. FERREIRA K. D. HAMMOND D. A. GILBERT |
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| Affiliation: | 1. Research Center in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sports, University of Porto (FADEUP) and Laboratory for Integrative and Translational Research in Population Health (ITR), Porto, Portugal;2. LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Aveiro, Portugal;3. Centre for Research and Technology of Agro Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Quinta de Prados, Vila Real, Portugal;4. TOXRUN – Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL, Gandra, Portugal;5. Physiology and Anatomy, German Sport University, Cologne, Germany;1. LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Aveiro, Portugal;2. iBiMED, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal;3. Newborn Screening, Metabolism and Genetic Unit, Human Genetics Department, National Institute of Health Dr. Ricardo Jorge, Porto, Portugal;4. Department of Pathological, Cytological and Thanatological Anatomy, School of Health, Polytechnic Institute of Porto, Porto, Portugal;5. LAQV, Department of Chemistry, University of Aveiro, Aveiro, Portugal;1. Department of Biology and Biotechnology, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy;2. Department of Pharmacy and Biotechnology, University of Bologna, Viale Risorgimento 4, 40136, Bologna, Italy;3. Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Via S. Giacomo 14, 40126, Bologna, Italy;4. Department of Medicine, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, Italy;5. Department of Industrial Chemistry Toso Montanaro, University of Bologna, Viale Risorgimento 4, 40136, Bologna, Italy;6. CSGI, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Firenze, Italy |
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| Abstract: | Early studies, using kinetic methods, suggested that the isozyme pattern of lactate dehydrogenase in various cells oscillated with time. More recent electrophoretic studies on murine erythroleukaemic cells (which exhibit only one isozyme) indicated very high frequency variations (period 2min or less) in the amount of the lone active isozyme. We now show that in HL60 cells, the activity stain intensities of the two major isozyme bands both oscillate but the temporal variations are distinct. As with other cellular rhythms, each of the two periodicities seem to be modulated in cyclic fashion with respect to period, amplitude and mean levels, the periods of both the primary and modulating rhythms being of the order of 10–15min or probably much less. |
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| Keywords: | lactate dehydrogenase isozymes oscillation independent rhythms |
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