ALBUMIN CAN REVERSE THE RELEASE OF POTASSIUM FROM HUMAN ERYTHROCYTES TREATED WITH THE NON-IONIC DETERGENT,BRIJ 58 |
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Authors: | PETER BOGNER,DENYS N. WHEATLEY,CSABA BORB LY,ATTILA MISETA |
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Affiliation: | PETER BOGNER,DENYS N. WHEATLEY,CSABA BORBÉLY,ATTILA MISETA |
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Abstract: | Exchange of erythrocyte intracellular (i/c) K+for extracellular (e/c) Na+in human erythrocytes treated with sub-CMC concentrations of the non-ionic detergent Brij 58 can be stopped by reincubation in serum or albumin containing solutions. The progressive equilibration of the K+contents of detergent-treated human erythrocytes with the incubation medium was reversed by an albumin-mediated withdrawal of detergent molecules from the cell. Re-establishment of near normal [K+] in terms of K+/kg water proceeds in two ways: (i) a metabolism-dependent net accumulation of K+ions; and (ii) a metabolism-independent shrinkage of erythrocytes, this being the more significant factor. |
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Keywords: | human erythrocyte potassium detergents albumin membranes metabolism |
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