适合飞虱虫疠霉菌丝生产的液体培养基组分及发酵条件

蚜虫、飞虱、叶蝉等是威胁农业生产的刺吸式口器害虫,长期以来依赖化学农药防治,严重影响农产品的安全性和农业生态环境.探讨这类害虫的生物防治途径,减轻农产品化学农药的残留污染,一直受到国内外学者的关注.虫霉(En-tomophthorales)的许多种类具有优异的诱病杀虫能力,尤其通过主动弹射孢子而在害虫种群中高强度流行的特征,可在短期内迅速压低虫口密度,是极其重要的杀虫微生物资源[1～3].

Optional Growth Medium and Conditions for Mass Production of Pandora delphacis Mycelia in Submerged Culture

The entomophthoraceous fungi are important microbial control agents for insect control but their mass production is usually difficult and expensive. To produce in large quantity the mycelia of the entomophthoraceous fungus, Pandora delphacis (isolate F95129), this study was aimed at replacing expensive components of liquid medium that is usually used in laboratory only with cheap materials easily available. Based on comparative experiments with primary components of several media designed and optional conditions for submerged culture, an appropriate medium was recognized that included(per liter) 10 g of homemade yeast extract, 10 g industrial fish meal, 10 g wheat bran, 15 g corn meal, 1.0 g KH2PO4, 3.0 g NH4NO3, and 0.25 g MgSO(4).7H2O. The optional conditions for submerged culture was 25-30 degrees C, initial pH 6.5, and 40% (V/V) of flask filled with the medium and 10% of initial inoculum (liquid culture containing mycelia) for inoculation. Using the selected medium and the conditions considered, 48 h culture resulted in a considerably high yield of dry mycelia(> 25 mg/mL) with desirable capacity of spore production.