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Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species |
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| Authors: | A. Akkak V. Scariot D. Torello Marinoni P. Boccacci C. Beltramo R. Botta |
| Affiliation: | (1) Dipartimento Scienze Agro-Ambientali, Chimica e Difesa Vegetale, Università degli Studi di Foggia, Via Napoli 25, I-71100 Foggia, Italy;(2) Dipartimento di Agronomia, Selvicoltura e Gestione del Territorio, Università di Torino, Via Leonardo da Vinci 44, I-10095 Grugliasco, Italy;(3) Dipartimento di Colture Arboree, Università di Torino, Via Leonardo da Vinci 44, I-10095 Grugliasco, Italy |
| Abstract: | Forty one simple sequence repeats were isolated from two microsatellite enriched libraries of date palm (Phoenix dactylifera L.). After screening, 17 selected microsatellite loci were characterized and evaluated on a set of 31 cultivars and clones from Algerian and Californian germplasm. All primer pairs produced an amplification product of the expected size and detected high polymorphism among the analysed samples. These nuclear simple sequence repeat (SSR) markers are expected to be a very effective tool for evaluating genetic diversity in date palm germplasm. Acrosstaxa amplification showed the usefulness of most SSR markers in 14 other species across the genus Phoenix. |
| Keywords: | KeywordHeading" >Additional across-taxa transferability date palm simple sequence repeats |
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