Simplified ultraviolet liquid chromatographic method for determination of sertindole, dehydrosertindole and norsertindole, in human plasma |
| |
Authors: | Canal-Raffin Mireille Déridet Evelyne Titier Karine Frakra Eric Molimard Mathieu Moore Nicholas |
| |
Institution: | Department of Clinical Pharmacology and Toxicology, EA-3676, IFR 99, University Victor Segalen Bordeaux 2, Bat IA, 33076 Bordeaux Cedex, France. |
| |
Abstract: | An ultra-violet high-performance liquid chromatographic method was developed for the determination of sertindole, an atypical antipsychotic drug and its main metabolites dehydrosertindole and norsertindole, in human plasma. With a small sample volume, after a single-step liquid-liquid extraction, the compounds were separated on a reversed-phase XTerra RP(18) column, eluted with 45% of acetonitrile and 55% of ammonium acetate buffer (0.05 M, adjusted pH 8) and detected at 256 nm within 11 min. This method shows a good linearity for plasma concentration between 5-100 ng/ml and 100-1000 ng/ml, a good precision (inter and intra day CV < 11%) and a good inter-assay accuracy (bias < 11%). The limit of quantification concentration was 5 ng/ml. The absolute recovery of sertindole was higher than 99%. This rapid and sensitive method could be used for therapeutic drug monitoring as well as for overdose management. |
| |
Keywords: | Sertindole Human plasma Liquid–liquid extraction Drug monitoring Overdose |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|