Maturation of adult rat peritoneal and mesenteric mast cells |
| |
Authors: | Valmor O Mendonça Ithamar Vugman Maria Celia Jamur |
| |
Institution: | (1) Departamento de Morfologia, Faculdade de Medicina, Ribeirão Preto, São Paulo, Brasil;(2) Departamento de Morfologia, Faculdade de Medicina, 14100 Ribeirão Preto, SP, Brasil;(3) Present address: Departamento de Ciências Morfológicas, Fundação Universidade do Rio Grande, Brasil;(4) Present address: Departamento de Ciências Morfológicas, Universidade Federal do Parana. Fellow of CAPES, Brasil |
| |
Abstract: | Summary Repopulation and maturation of rat mesenteric and peritoneal mast cells were studied after mast cell depletion by intraperitoneal injection of distilled water. Immature mast cells were first identified in the mesentery and peritoneal fluid 5 and 6 days, respectively, after water injection. The most immature mast cells that could be identified contained a few orthochromatic granules. Upon maturation, the granules became metachromatic and increased in size and number. Heparin, revealed by toluidine blue staining and berberine sulfate fluorescence, appeared simultaneously with orthophthaldialdehyde (OPT)-induced histamine fluorescence. Paraformaldehyde-induced serotonin fluorescence appeared somewhat later. Repopulation of mesentery and peritoneal fluid by mast cells seemed to be independent of each other and to occur from undifferentiated precursor cells. |
| |
Keywords: | Mast cells Granule maturation Histamine Serotonin Heparin Rat |
本文献已被 SpringerLink 等数据库收录! |
|