Matrix-associated DNA from maize is enriched in repetitive sequences |
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| Authors: | Lubomir M. Stoilov Valeria Mirkova Jordanka Zlatanova Lalio Djondjurov |
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| Affiliation: | (1) Institute of Genetics, Bulgarian Academy of Sciences, PO Box 96, 1113 Sofia, Bulgaria;(2) Institute of Molecular Biology, Bulgarian Academy of Sciences, PO Box 96, 1113 Sofia, Bulgaria |
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| Abstract: | In order to elucidate some features of the topological organization of DNA within the plant nucleus, DNA fragments involved in the attachment of the DNA loops to the nuclear matrix in maize were studied. The matrix-associated DNA from dry embryo and meristematic cells after extensive digestion with DNase I and high salt treatment was about 2% of the total DNA, sized within the range of 50 and 250 bp. This DNA was found to be enriched in repetitive DNA sequences, both for nuclei from dry embryo and meristematic cells. The loop size of the DNA in cells of Zea mays appeared to be between 5 and 25 kbp.Abbreviations EDTA Diamino-ethanetetraacetic acid - EtBr Ethidium bromide - LIS Lithium diiodosalicylate - PMSF Phenylmethylsulfonyl fluoride - SDS Sodium dodecyl sulfate |
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| Keywords: | Zea mays Matrix-associated DNA repetitive sequences DNA loops |
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