| 厚荚相思RNA提取方法的建立与优化 |
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| 引用本文： | 姚冬琳,刘伟娜,杨明嘉,郭惠红,金一,谢响明. 厚荚相思RNA提取方法的建立与优化[J]. 生命科学研究, 2012, 16(4): 301-306 |
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| 作者姓名： | 姚冬琳 刘伟娜 杨明嘉 郭惠红 金一 谢响明 |
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| 作者单位： | 1. 北京林业大学生物科学与技术学院,中国北京,100083
2. 中国科学院生态环境研究中心,中国北京,100085 |
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| 基金项目： | 国家自然科学基金资助项目，北京林业大学青年科技启动基金资助项目 |
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| 摘 要： |
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| 关 键 词： | 厚荚相思 RNA提取 方法优化 |
The Establishment and Optimization of RNA Isolation in Acacia crassicarpa |
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| YAO Dong-lin , LIU Wei-na , YANG Ming-jia , GUO Hui-hong , JIN Yi , XIE Xiang-ming. The Establishment and Optimization of RNA Isolation in Acacia crassicarpa[J]. Life Science Research, 2012, 16(4): 301-306 |
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| Authors: | YAO Dong-lin LIU Wei-na YANG Ming-jia GUO Hui-hong JIN Yi XIE Xiang-ming |
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| Affiliation: | 1(1.College of Biological Science and Biotechnology,Beijing Forestry University,Beijing 100083.China; 2.Research Center for Eco-Envionmental Sciences,Chinese Academy of Sciences,Beijing 100085,China) |
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| Abstract: | Extraction of high-quality RNA from the phyllode of Acacia crassicarpa is very difficult due to high levels of tannin,phenols,pigment,cellulose polysaccharide that bind or coprecipitate with RNA.There-fore,the preferred RNA isolation protocols were optimized from the phyllode of Acacia crassicarpa,compar-ing the Trizol method,improved hot-borate methods,CTAB and RNA isolation kit combined with ultrasonic method.It was found that the RNA isolation kit combined with 20 MHz ultrasonic processor(puls on 5 s,puls off 5 s) was optimal for the RNA isolation from Acacia crassicarpa.The extracted total RNA with this method had high integrity,the ratio of A260/A280 was in the range of 1.82～1.98,and had been successfully used for RT-PCR experiment. |
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| Keywords: | Acacia crassicarpa RNA isolation optimized methods |
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