pepM is an essential gene in Salmonella typhimurium. |
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Authors: | C G Miller A M Kukral J L Miller N R Movva |
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Affiliation: | Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio 44106. |
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Abstract: | The pepM gene of Salmonella typhimurium codes for a methionine-specific aminopeptidase that removes N-terminal methionine residues from proteins. This gene was inactivated in vitro by the insertion of a DNA fragment coding for kanamycin resistance. The inactivated gene could not replace the wild-type chromosomal pepM gene unless another functional copy was present in the cell. The lethal effect of the pepM insertion was not a result of polarity on any gene downstream, nor was it affected by the presence or absence of other peptidases. |
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