Terminal differentiation of human promyelocytic leukaemia cells, HL-60, during the G1 period |
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Authors: | Matsuhisa T |
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Institution: | Department of Cell Biology, The Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3, Nakamichi, Higashinari, Osaka, 537-8511, Japan. |
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Abstract: | Human promyelocytic leukaemic cells, HL-60, arrested in mitosis by nocodazole were released in the presence of 1alpha,25-dihydroxyvitamin D3 and thymidine or hydroxyurea. Cells moved from early G1 period to the G1/S boundary and differentiated. Furthermore, cells arrested at the G1/S boundary by double thymidine block were released, with 1alpha,25-dihydroxyvitamin D3 being added at the end of DNA synthesis. Under the latter conditions, differentiated cells developed, indicating that DNA synthesis is not required for cell differentiation. |
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Keywords: | cell differentiation vitamin D3 cell cycle |
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