几种植物组织及其原生质体过氧化物酶同工酶的比较研究

对马尾松幼苗子叶和胚轴、甘蔗、小麦、烟草、黄花菜等幼叶及其原生质体的过氧化物酶同工酶(POI)分别作比较研究。观察到凡经纤维素酶处理的各植物组织POI酶带数均多于未经纤维素酶处理的组织的酶带数;除个别例外,后者一般又比无壁原生质体的酶带数多。此种差异随植株生长年龄而增大,表明植物组织内大部分POI主要存于质外体中。

COMPARATIVE STUDIES ON PEROXIDASE ISOZYME PATTERNS IN THE TISSUES AND PROTOPLASTS OF FIVE SPECIES

By using polycrylamide gel electrophoresis method, peroxidase isozyme (POI) from the leaf tissues and protoplasts of five species, i. e., cotyledon and plumular axis of masson-pine (Pinus massoniana Lamb.) and leaves of wheat (Triticum aestivum L.), sugar-cane (Saccharum officnarum), tobacco (Nicotiana tabacum) and daylily (Hemerocallis fulva) were obtained:POI of cotyledon tissues of masson-pine that are digested and that are not digested by cellulase had 8 and 5 bands respectively, but their isolated protoplasts had 3 bands. POI of plumular axis of masson-pine that are digested by cellulase had 10 bands, but the POI patterns of that are not digested by cellulase and their isolated protoplasts are identical and all had 8 bands.POI of leaf tissues of wheat seedling that are digested cellulase had 9 bands, but the POI patterns of that are not digested by cellulase and their isolated protoplsats are identical and all had 4 bands.POI patterns of young leaf tissues of sugar-cane at different growth phase are difference. POI of leaf tissues that are digested and that are not digested by cellulase all had 3 bands, but their isolated protoplasts had only 1 bands on September the twenty-seventh, POI of leaf tissues that are digested and that are not digested by cellulase had 6 and 5 bands respectively, and their isolated protoplasts had 3 bands on November the fourteenth. POI of leaf tissues that are digested and that are not digested by cellulase had 9 and 7 bands respectively, and their isolated protoplasts had 4 bands on December the fourth.POI of leaf tissues of tobacco that are not digested by cellulase and their isolated protoplasts had 9 and 3 bands respectively.POI of young leaf tissues of daylily that are not digested by cellulase and their isolated protoplasts had 7 and 3 bands respectively.The above mentioned results showed most of POI were located on the apoplast, and POI isolated from non-cell wall protoplasts are only represent components of POI in the symplast.