4-PA对BEL-7402细胞的生长调控和诱导凋亡作用的研究

应用ＭＴＴ、流式细胞术研究了新型肿瘤诱导分化剂４－ＰＡ对人肝癌细胞系ＢＥＬ－７４０２细胞的生长调控、诱导调亡作用。结果表明：４－ＰＡ对ＢＥＬ－７４０２细胞增殖抑制的ＩＣ５０为４－９ｍｍｏｌ／Ｌ，随着作用时间的增加，ＩＣ５０增加。作用效果迅速，在０．５小时就有效地抑制了细胞增殖，抑制率随４－ＰＡ浓度的增加和作用时间的处长而增加，在达到最大抑制率后出现饱和。流式细胞仪对细胞周期和细胞调亡的分析发现：４

THE EFFECT OF 4-PA ON THE GROWTH AND APOPTOSIS OF BEL-7402 CELLS

The present study was conducted to define the effect of 4-PA,a new antitumor differentiation inducer, on the proliferation and apoptosis of BEL-7402 cells was studied. The IC50 of 4-PA on the proliferation of BEL-7402 ranges from 4 to 9mmol/L and increases with time going on. The effect of 4-PA is as short as 0.5 hour versus 12hours of 5-FU and DDP. The inhibitory rate increases with time and 4-PA concentration, and gets saturated after reaching the maximum. It indicates that 4-PA has cell circle speciality on cell proliferation. The rapid effect is related to its molecule small in site, easy to penetrate cell membrane and affecting gene expression. The results of cell circle and apoptosis by flow cytometry show that 4-PA has a strong inhibition and reversal effect on the cell proliferating phases(S and G2 phases),and makes the cells stop mainly in G1 phase.The percentage of cell apoptosis increases with treating time and 4-PA concentration. The apoptosis cells comes mainly from cell proliferating phases(S and G2 phases).