4-PA对BEL-7402细胞的生长调控和诱导凋亡作用的研究 |
| |
引用本文: | 蔡国友,朱建平,沈蔚茵,沈子威. 4-PA对BEL-7402细胞的生长调控和诱导凋亡作用的研究[J]. 生物物理学报, 1999, 15(4): 661-666 |
| |
作者姓名: | 蔡国友 朱建平 沈蔚茵 沈子威 |
| |
作者单位: | [1]清华大学生物系生物膜与膜生物工程国家重点实验室,北京 [2]清华大学生物系生物膜与膜生物工程国家重点实验室,北 |
| |
摘 要: | 应用MTT、流式细胞术研究了新型肿瘤诱导分化剂4-PA对人肝癌细胞系BEL-7402细胞的生长调控、诱导调亡作用。结果表明:4-PA对BEL-7402细胞增殖抑制的IC50为4-9mmol/L,随着作用时间的增加,IC50增加。作用效果迅速,在0.5小时就有效地抑制了细胞增殖,抑制率随4-PA浓度的增加和作用时间的处长而增加,在达到最大抑制率后出现饱和。流式细胞仪对细胞周期和细胞调亡的分析发现:4
|
关 键 词: | 4-PA BEL-7402 细胞增殖 细胞调亡 |
THE EFFECT OF 4-PA ON THE GROWTH AND APOPTOSIS OF BEL-7402 CELLS |
| |
Abstract: | The present study was conducted to define the effect of 4-PA,a new antitumor differentiation inducer, on the proliferation and apoptosis of BEL-7402 cells was studied. The IC50 of 4-PA on the proliferation of BEL-7402 ranges from 4 to 9mmol/L and increases with time going on. The effect of 4-PA is as short as 0.5 hour versus 12hours of 5-FU and DDP. The inhibitory rate increases with time and 4-PA concentration, and gets saturated after reaching the maximum. It indicates that 4-PA has cell circle speciality on cell proliferation. The rapid effect is related to its molecule small in site, easy to penetrate cell membrane and affecting gene expression. The results of cell circle and apoptosis by flow cytometry show that 4-PA has a strong inhibition and reversal effect on the cell proliferating phases(S and G2 phases),and makes the cells stop mainly in G1 phase.The percentage of cell apoptosis increases with treating time and 4-PA concentration. The apoptosis cells comes mainly from cell proliferating phases(S and G2 phases). |
| |
Keywords: | PA BEL-7402 Cell proliferation Cell apoptosis |
本文献已被 维普 等数据库收录! |
| 点击此处可从《生物物理学报》浏览原始摘要信息 |
|
点击此处可从《生物物理学报》下载全文 |
|